Cột HPLC Hamilton PRP-1, 4.1 mm x 150 mm PN: 79444
Cột tách Vitamin E( bao gồm các đồng phân của tecopherol) 

Hãng sản xuất: Hamilton

 

Chromatographic Conditions  
Compound Classification
 Tocopherol
Gradient 80-100% B (0-10 minutes), 100% B (10-12 minutes)
Temperature Ambient
Injection Volume 5 µL
Detection UV at 292 nm
Column Dimensions 150 x 4.1 mm
Eluent A Ammonium Acetate
Eluent A Concentration 20 mM
Eluent B 1:1 Acetonitrile/Tetrahydrofuran

Flow Rate

 

Compound:

1Δ-Tocopherol

2 β + γ-Tocopherol

3 α-Tocopherol

1.0 mL/min